PEI及其共聚物的胶囊化siRNA在肿瘤基因治疗中的研究进展(6)

中国海洋药物．２００２．２ｌｆ３）：５４．５６．【３１蒋挺大．壳聚糖ＩＭ】．北京：化学工业出版社．２００ｌ：１２．３２．【４】陈夫山。谢来苏．用胶体滴定法测定聚合物的电荷【Ｊ】．中国造纸．

加Ｉｏｏ．１９（２）：３０ ３４．

【５】ＫａｎｅｋｏＭ，ｌｎｏｕｅＹ．ＴｏｋｕｒａＳ．ＲｅｆｒａｃｔｉｖｅＩｎｄｅｘＩｎｃｒｅｍｅｎｔｓｏｆ

Ｐｒｏｔｅｏｇｌｙｃａｎ［Ｊ１．ＲｅｐＰｒｏｇＰｏｌｙｍＰｈｙｓＪｐｎ．１９８４．２７：７５９－７６２．【６１曾德芳．马甲益，袁继租．Ｎ．０一羧甲摹壳聚糖制备工艺的优化研

究ｌＪＪ．武汉理］：大学学报．２００５．２７（６）：Ｊ５．１８．

川唐振兴．钱俊青．石陆娥．羧甲蓦壳聚糖制备工艺的研究Ⅲ．化工技

术与开发．２００４．３３（２）：４．７．【８Ｊ

ＭｕｚｚａｒｅｉｌｉＲＡ．１ｉａｒ／Ｐ．ＰｅｔｒａｍｌｏＭ．Ｓｏｌｕｂｉｌｉｔｙａｎｄ

ｓｔｒｕｃｔｕｒｅ

浓度和温度而造成壳聚糖分子的降解；但加入硼氢

化钠不能过多，否则不利于脱乙酰反应的进行。４结论

Ｎ，Ｏ．ＣＭＣ制备的最佳工艺过程：壳聚糖５ｇ，异丙醇溶胀，１０

ｍｏｌ／Ｌ

ＮａＯＨ溶液６２．５ｍＬ在一ｌＯ℃碱

化１２ｈ．氯乙酸／壳聚糖（ｍｏｌ／ｍ０１）＝６：ｌ，反应温度为６０℃，反应时间为５

ｈ。

ｏｆＮ

ｃａｒｂｏｘｙｍｅｔｈｙｌｃｈｉｔｏｓａｎ［Ｊ］．ＩｎｔＪＢｉｏｌＭａｃｒｏｍｏｌ，１９９４．１６（４）：１７７－１８０．【９】陈鲁生．李赢霖．０－羧甲摹壳聚糖制备条件的研究【ＪＪ．山东师范

大学学报：自然科学版．１９９９．１４ｆＩ）：４２．４３．

【１０１邵健．杨字民．水溶性Ｏ．羧甲基壳聚糖的制备【Ｊ１．南通医学院学

报．２０００．２０（２）：１５５－１５６．

【ｌｌ】李治，刘晓非．Ｏ．羧甲基壳聚糖抗菌性的研究【Ｊ１．日用化学工业．

２０００．３０（３）：１０－１１．

（收编日期：２０１０－０９－０２）

Ｎ．ＣＭＣ制备的最佳工艺过程：乙醛酸，壳聚糖

（摩尔比）＝１．２，常温（２０．４０℃）反应２ｈ．调节体系ｐＨ值为８一ｌＯ，分批加入１０％硼氢化钠常温（２０－４０℃）反应２ｈ。

Ｏ．ＣＭＣ制备的最佳Ｔ艺过程：甲壳素异丙醇溶

万方数据

PEI及其共聚物的胶囊化siRNA在肿瘤基因治疗中的研究进展

作者：作者单位：

史秋生， 段友容， 杜联芳， SHI Qiu-sheng， DUAN You-rong， DU Lian-fang

史秋生,杜联芳,SHI Qiu-sheng,DU Lian-fang(上海交通大学附属第一人民医院超声科分子影像研究室,200080)， 段友容,DUAN You-rong(上海交通大学肿瘤研究所药物递送研究室,200032)

国际生物医学工程杂志

INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING2011,34(1)

刊名：英文刊名：年，卷(期)：

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