中华眼镜蛇心脏毒素(CardiotoxinⅢ)的克隆及其在大肠杆菌和酵母

心脏毒素cardiotoxinⅢ(CTXⅢ)是中华眼镜蛇毒主要组分之一,根据Genbank中已有的CTXs序列设计特异性引物,从中华眼镜蛇毒中克隆出208bp的CTXⅢ片段,并将该基因片段分别克隆至大肠杆菌His—patch ThioredoxinB载体中,重组CTXⅢ经渗透休克分泌至胞外。将CTXⅢ基因插入酵母pPIC9K分泌型表达载体重组表达,N端带有3个氨基酸(GYT)残基的重组CTXⅢ(rCTXⅢ)分泌量达9.5mg／L,

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