氧化应激诱导HepG2肝癌细胞凋亡的研究

目的：直接暴露细胞于活性氧能诱导发生凋亡,本文研究氧化应激诱导HepG2肝癌细胞的死亡及其机制。方法：暴露细胞于2mmoL／L过氧化氢产生氧化应激,用DNA凝胶电泳检测细胞凋亡,用荧光染色法检测细胞线粒体膜电位变化,Western blotting检测细胞浆中细胞色素c变化,fluorometric assay kit检测caspase活性变化。结果：氧化应激作用于HepG2细胞后12h开始发生凋亡;氧化应激作用后4

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