生信实验 模拟卷(1)

试题编号：

重庆邮电大学2010-2011学年2学期 《生物信息学及实验》试卷（期末）（A卷）（闭卷）

题 号 得 分 评卷人

一、名词解释题（本大题共10小题，每小题3分，共30分） 1. bioinformatics 2. Entrez 3.SWISS-MODE

4. molecular phylogenetic tree 5. neighbor-joining method

6.MEGA(Molecular Evolutionary Genetics Analysis) 7.ORF 8. CpG island 9.structure domain 10.linux operating system

二、简答题（本大题共6小题，每小题5分，共30分） 1. Why do biological scientists search DNA databases? 2.用UPGMA重建系统发生树，距离矩阵如下： 物种 B C D E

3.NCBI维护的核苷酸数据库由哪几部分组成的，其主要内容是什么？ 4. 写出BLAST算法的流程。

5. 写出同源模建法预测蛋白质三级结构的方法流程及原理

6. What is the difference between Molecular Dynamics and Molecular Mechanism?

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A 3 6 9 12 B - 5 9 11 C - - 10 13 D - - - 9 一 二 三 四 五 六 七 八 总 分

三、翻译题（本大题共2小题，每小题10分，共20分）

1. Bioinformatics consists of a computational approach to biomedical information

management and analysis. It is being used increasingly as a component of research within both academic and industrial settings and is becoming integrated into both undergraduate and postgraduate curricula. The new generation of biology graduates is emerging with experience in using bioinformatics resources and, in some cases, programming skills.

The National Center for Biotechnology Information (NCBI) is one of the world's premier Web sites for biomedical and bioinformatics research. Based within the National Library of Medicine at the National Institutes of Health, USA, the NCBI hosts many databases used by biomedical and research professionals. The services include PubMed, the bibliographic database; GenBank, the nucleotide sequence database; and the BLAST algorithm for sequence comparison, among many others.

2. The GenBank sequence database is an annotated collection of all publicly available

nucleotide sequences and their protein translations. This database is produced at National Center for Biotechnology Information (NCBI) as part of an international collaboration with the European Molecular Biology Laboratory (EMBL) Data Library from the European Bioinformatics Institute (EBI) and the DNA Data Bank of Japan (DDBJ). GenBank and its collaborators receive sequences produced in laboratories throughout the world from more than 100,000 distinct organisms. GenBank continues to grow at an exponential rate, doubling every 10 months. Release 134, produced in February 2003, contained over 29.3 billion

nucleotide bases in more than 23.0 million sequences. GenBank is built by direct submissions from individual laboratories, as well as from bulk submissions from large-scale sequencing centers.

四、设计题题（本大题共2小题，每小题10分，共20分）

1．利用Smith-Waterman算法，为下列两条序列的局部比对建立部分比对打分表(匹配得分= +1分，失配得分= 0分，空位罚分= -1分)： ACGTATCGCGTATA GATGCTCTCGGAAA

两序列间最佳局部比对是什么？

2．下面是某基因的一段序列，请设计一对引物，扩增箭头所示片段，要求能够上游利

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用BamH I（GGATTC）和下游利用EcoR I（GAATTC）连接到克隆载体上。

GCCCTTGGACTGGGACTTAGCCCCATTGGCCCCCTGGTTCTCAGGCCTTCAGCCTGAGTTACACCACCAGCTTTCTTGGGTCTCAGTTTGCAAATGGCAGATCGTGGGACTTCGCAAACACCATCATCTTGTAAGCCCATTCCTCCTAATCTTCTTTTATATACACACCAATTGGTTCTGTTTCTCTGGAAAACCTGATTAATAGACCTGAGGAATGAAACTGTAACCTCTCAGTGGGTTCTTCTTGCCTGAGCCTGGCGTCCGTGTGGCTGGCGCGGAG GATGTGGACGCTGCGGAGCCCGCTCACCCGCTCCCTGTACGTGAAGGAGAACCACCAGTGGTATGTGTGCAACAGAGAGAAATTATGCGAATCACTCCAGGCTGTCTTTGTTCAGAGTTACCTTGATCAAGGAACACAGATCTTCTTAAACAACAGCATTGAGAAATCGGGCTGGCTATTTATCCAATTATATCATTCTTTTGTGTCATCTGTTTTTAGCCTGTTTATGTCTAGAACATCTATCAATGGGTTGCTAGGAAGAGGCTCAATGTTTGTGTTTTCACCAGATCAGTTTCAGAGACTGCTTAAAATTAATCCAGACTGGAAAACCCACAGACTTCTTGATTTAGGTGCTGGAGATGGAGAAGTCACAAAAATCATGAGCCCTCATTTTGAAGAAATCTATGCCACTGAGCTTTCTGAAACTATGATATGGCAGCTTCAGAAAAAGAAATACAGAGTCCTTGGTATAAATGAATGGCAGAATACG

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