ECM培养基 Sciencell 1001

Endothelial Cell Medium

(ECM)

Catalog Number: 1001

Product Description

Endothelial cell medium (ECM) is a complete medium designed for optimal growth of normal human microvascular endothelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human microvascular endothelial cells in vitro.

Components

ECM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of endothelial cell growth supplement (ECGS, Cat. No. 1052) and 5 ml of penicillin/streptomycin solution (P/S, Cat. No. 0503).

Product Use

in vitro diagnostic procedures.

Storage

Store the basal medium at 4oC, the ECGS, the FBS and the P/S solution at -20oC. Protect from light.

Shipping

Gel ice.

Prepare for use

Thaw ECGS, FBS and P/S solution at 37oC. Gently tilt the ECGS tube several times during thawing to help the contents dissolve. Make sure the contents of the supplement are completely dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add ECGS, FBS and P/S solution into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of this medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37oC. When stored in the dark at 4oC, the reconstituted medium is stable for one month.

Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.