亚粒径阳离子交换色谱

赛分科技离子交换色谱产品具有多种规格的粒径可供选择,小粒径填料产品对样品分离达到高分辨率、稳定的完美效果。

赛分科技离子交换色谱产品具有多种规格的粒径可供选择,小粒径填料产品对样品分离达到高分辨率、稳定的完美效果。

Content

Introduction (1)

Technical specifications of Proteomix® NP1.7 and Antibodix? NP1.7 (2)

Quality Control Test for Proteomix® SCX NP1.7 4.6 x 30 mm (3)

Quality Control Test for Proteomix® SCX NP1.7 4.6 x 50 mm (3)

Quality Control Test for Proteomix® WCX NP1.7 4.6 x 50 mm (4)

Quality Control Test for Antibodix? WCX NP5 4.6 x 250 mm (5)

Particle Size Comparison for Proteomix® SCX 4.6 x 50 mm (6)

Particle Size Comparison for Proteomix® WCX 4.6 x 50 mm (6)

Lot to Lot Reproducibility for Proteomix® SCX 4.6 x 50 mm (7)

Lot to Lot Reproducibility for Proteomix® WCX 4.6 x 50 mm (7)

Column Lifetime for Proteomix® SCX 4.6 x 50 mm (8)

Column Lifetime for Proteomix® WCX 4.6 x 50 mm (8)

Proteomix® SCX and WCX NP1.7 Applications (9)

MAb separation on Proteomix® SCX NP1.7 4.6 x 100 mm vs. NP5 4.6 x 250 mm . 9 MAb separation on Proteomix® SCX NP1.7 vs. Proteomix® SCX NP5 (4.6 x 100 mm) (9)

Separation of BSA Digest on Proteomix® SCX NP1.7 2.1 x 30 mm (10)

Separation of Peptides on Proteomix® SCX NP1.7 2.1 x 30 mm (10)

Gradient Optimization for Peptide Separation on Proteomix® SCX NP1.7 (11)

Particle Size Comparison for the Separation on Peptides on Proteomix® SCX (11)

Shorter Run Time for Peptide Separation on Proteomix® SCX NP1.7 (12)

Zoomed View of Short Run for Peptide Separation on Proteomix® SCX NP1.7 (12)

Analysis of Fab and Fc Fragments on Proteomix® WCX NP1.7 (13)

Antibodix? WCX NP1.7 Applications (14)

Particle Size Comparison for the Separation of MAb on Antibodix? WCX (14)

MAb Loading Study on Antibodix? WCX NP1.7 4.6 x 100 mm (14)

Particle Size Comparison for the Separation of MAb on Antibodix? WCX (15)

Gradient Optimization for MAb Separation on Antibodix? WCX NP1.7 (15)

Troubleshooting (16)

Ordering Information (17)

赛分科技离子交换色谱产品具有多种规格的粒径可供选择,小粒径填料产品对样品分离达到高分辨率、稳定的完美效果。

1-877-SEPAX-US Introduction

Figure 1. General structure for Sepax’s Cation Exchange Phases.

Key features of Proteomix® and Antibodix? phases

1

赛分科技离子交换色谱产品具有多种规格的粒径可供选择,小粒径填料产品对样品分离达到高分辨率、稳定的完美效果。

1-877-SEPAX-US Technical specifications of Proteomix® CEX and Antibodix? WCX

2

赛分科技离子交换色谱产品具有多种规格的粒径可供选择,小粒径填料产品对样品分离达到高分辨率、稳定的完美效果。

1-877-SEPAX-US

3

Quality Control Test for Proteomix ® SCX NP1.7 4.6 x 30 mm

Figure 2. A standard quality control test on a Proteomix ® SCX NP1.7 4.6 x 30 mm. Mobile phase A: 20 mM sodium phosphate buffer pH 6.0 and B: A + 1.0 M NaCl. The gradient was 0-50% B in 5 minutes with a 15 minute prewash. Flow rate was 0.2 mL/min. UV detection was set at 214 nm. 5 µL of sample was injected and the sample is a mixture of Ribonuclease A, Cytochrome C and Lysozyme (1 mg/mL each).

Quality Control Test for Proteomix ® SCX NP1.7 4.6 x 50 mm

Figure 3. A standard quality control test on a Proteomix ® SCX NP1.7 4.6 x 50 mm. Mobile phase A: 20 mM sodium phosphate buffer pH 6.0 and B: A + 1.0 M NaCl. The gradient was 0-75% B in 25 minutes with a 15 minute prewash. Flow rate was 0.75 mL/min. UV detection was set at 214 nm. 5 µL of sample was injected and the sample is a mixture of Ribonuclease A, Cytochrome C and Lysozyme (1 mg/mL each).

赛分科技离子交换色谱产品具有多种规格的粒径可供选择,小粒径填料产品对样品分离达到高分辨率、稳定的完美效果。

1-877-SEPAX-US

4

Quality Control Test for Proteomix ® WCX NP1.7 4.6 x 50 mm

Figure 4. A standard quality control test on a Proteomix ® WCX NP1.7 4.6 x 50 mm. Mobile phase A: 20 mM sodium phosphate buffer pH 6.5 and B: A + 1.0 M NaCl. The gradient was 0-100% B in 25 minutes with a 15 minute prewash. Flow rate was 0.5 mL/min. UV detection was set at 214 nm. 5 µL of sample was injected and the sample is a mixture of Ribonuclease A, Cytochrome C and Lysozyme (1 mg/mL each).

赛分科技离子交换色谱产品具有多种规格的粒径可供选择,小粒径填料产品对样品分离达到高分辨率、稳定的完美效果。

1-877-SEPAX-US

5

Quality Control Test for Antibodix ? WCX NP1.7 4.6 x 50 mm

Figure 5. A standard quality control test on a Antibodix ?

WCX NP1.7 4.6 x 50 mm. Mobile phase A: 10 mM sodium phosphate buffer pH 6.0 and B: A + 1.0 M NaCl. The gradient was 10-100% B in 25 minutes with a 15 minute prewash. Flow rate was 0.5 mL/min. UV detection was set at 214 nm. 5 µL of sample was injected and the sample is a mixture of Ribonuclease A, Aprotinin and Lysozyme (1 mg/mL each).

赛分科技离子交换色谱产品具有多种规格的粒径可供选择,小粒径填料产品对样品分离达到高分辨率、稳定的完美效果。

1-877-SEPAX-US

6 Particle Size Comparison for Proteomix ® SCX 4.6 x 50 mm

Figure 6. A comparison of different particle sizes for Proteomix ® SCX NP 4.6 x 50 mm. Mobile phase A: 20 mM sodium phosphate buffer pH 6.0 and B: A + 1.0 M NaCl. The gradient was 0-75% B in 25 minutes with a 15 minute prewash. Flow rate was

0.75 mL/min. UV detection was set at 214 nm. 5 µL of sample was injected and the sample is a mixture of Ribonuclease A, Cytochrome C and Lysozyme (1 mg/mL each).< …… 此处隐藏：4733字，全部文档内容请下载后查看。喜欢就下载吧 ……