不同价态金属离子对BSA结构的影响(3)

65.1%

H.Zhangetal./SpectrochimicaActaPartA78 (2011) 523–527

527

403020

)

g10edm(θ0

-10-20-30190200210220230240250260270

Wavelength(nm)

40B

3020

)

g10edm(θ0-10-20

-30

190200210220230240250260270

Wavelength(nm)

Fig.6.CirculardichroismspectraofBSAwithdifferentconcentrationsofsodiumchlorideandaluminiumchloride.Conditions:c(BSA)=2.0×10 7mol/L,c(Na+,Al3+)/(1.0×10 6mol/L):0,1,100respectively;pH7.4.

Table4

Thecomparisonofthe -helixofBSAintheabsenceandpresenceofAl3+.System

-HelixcontentBSA

64.1%BSA+Na+(1.0×10 6mol/L)61.4%BSA+Na+(1.0×10 4mol/L)

59.2%

Wecan gureoutfromtheresultsabovethatthe -helixofBSAdecreasedclearlyinthepresenceofCu2+,inotherwords,theconformationofBSAwasdestroyedharmfullyintoxicityofCu2+.However,bothNa+andAl3+didlittleharmtothesecondarystruc-tureofBSA.5.Conclusions

Inthispaper,theeffectofmetalionsNa+,Cu2+,Al3+onBSAstruc-turehasbeeninvestigatedbymultiplespectroscopictechniquesincluding uorescence,RLS,synchronous uorescence,UV–visabsorptionandCDinvitroconditions.WecangettheconclusionthatthereislittlecorrelationbetweenionelectricchargesandthestructureofBSA.Speci cally,Na+didlittlein uencewiththestruc-tureofBSA,butcouldbeprotectivetowardsit.AndCu2+couldbindwithBSAintoalargerparticle,anddenatureitirreversibly,whichtaughtusthatCu2+cancausespeci clossoffunctionof

proteintotheproductionofharmfultoxiceffectonourphysicalbodies.Therefore,heavymetalpollutionintheenvironmentanddietisamajorhazardtohumanhealth,anditisofgreatimpor-tancetoimproveandprotectthetoxicityevaluationmechanism.Theresultsof uorescence,synchronous uorescence,ultravioletabsorptionandCDspectra,Al3+didnoharmtothestructureofBSA,butRLSofAl3+–BSAindicatedthatAl3+couldchangethesolutionenvironmentofBSAbyhydrolysisofAl3+inhighconcentrations.Acknowledgments

Thisworkwasaccomplishedunder nancialsupportofNatu-ralScienceFoundationofChina(20875055),theCultivationFundoftheKeyScienti candTechnicalInnovationProject,MinistryofEducationofChina(708058),andFoundationforExcellentYoungScientistsandKeyScience-TechnologyProjectinShandongProvince(2008GG10006012).AppendixA.Supplementarydata

Supplementarydataassociatedwiththisarticlecanbefound,intheonlineversion,atdoi:10.1016/j.saa.2010.11.021.References

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