常用酿酒酵母菌株基因型

Commonly used strains

information include:

used lab strains

identity between common lab strains S288C

Genotype: MATα SUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6

Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of , it is not a good strain for mitochondrial studies. It has an allelic variant of which increases petite frequency. S288C strains are gal2- and they do not use galactose anaerobically. The S288C genome was recently resequenced at the . References: (1986) Genetics 113:35-43.

BY4743

Genotype: MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15 ura3Δ0/ura3Δ0 Notes: Strain used in the , generated from a cross between BY4741 and BY4742, which are derived from S288C. As S288c, these strains have an allelic variant of which increases petite frequency. See Brachmann et al. reference for details. References: (1998) Yeast 14:115-32.

FY4

Genotype: MATa

Notes: Derived from S288C. References:

(1995) Yeast 11:53-55.

FY1679

Genotype: MATa/α ura3-52/ura3-52 trp1Δ63/TRP1 leu2Δ1/LEU2 his3Δ200/HIS3 GAL2/GAL

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. References: (1995) Yeast 11:53-55.

AB972

Genotype: MATα X2180-1B trp10 [rho 0]

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-trp1. References: (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.

A364A

Genotype: MATa ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIO

Notes: Used in the systematic sequencing project, the sequence stored in SGD. References: (1967) J. Bacteriol. 93:1662-1670.

XJ24-24a

Genotype: MATa ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2

Notes: Derived from, but not isogenic to, S288C References: (1979) Cell 18:309-319 DC5

Genotype: MATa leu2-3,112 his3-11,15 can1-11

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. References: (1979) Gene 8:121-133 X2180-1A

Genotype: MATa SUC2 mal mel gal2 CUP1

Notes:S288c spontaneously diploidized to give rise to X2180. The haploid segregants X2180-1a and X2180-1b were obtained from sporulated X2180

YNN216

Genotype: MATa/α ura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochre

Notes: Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin). References:

(1989) Genetics 122:19-27. YPH499

Genotype: MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1

Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note

thattrp1-Δ63, unlike trp1-Δ1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable marker.gal2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C. References:

(1989) Genetics 122:19-27.

YPH500

Genotype: MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1 Notes:MATα strain isogenic to YPH499 except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C. References:

Sikorski RS and Hieter P (1989) Genetics 122:19-27.

YPH501

Genotype: MATa/MATα ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-Δ63/trp1-Δ63 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1 Notes: a/α diploid isogenic to YPH499 and YPH500. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

References:

(1989) Genetics 122:19-27.

Sigma 1278B Notes: Used in pseudohyphal growth studies. about the sigma strains have been kindly provided by

Cora Styles. Sigma1278B background contain a nonsense mutation in RIM15, a G-to-T transversion at position 1216 that converts a Gly codon to an opal stop codon. This rim15 mutation interacts epistatically with mutations in certain other genes to affect colony morphology.

Annotation of the Sigma1278b genome and information about the systematic deletion collection can be found . SK1

Genotype: MATa/α HO gal2 cupS can1R BIO

Notes: Commonly used for studying sporulation or meiosis. Canavanine-resistant derivative.

The SK1 genome was sequenced at the . References: (1974) Bacteriol. 118: 8-14

CEN.PK (aka CEN.PK2)

Genotype: MATa/α ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his3 Δ1/his3 Δ1 MAL2-8C/MAL2-8C SUC2/SUC2

Notes: CEN.PK possesses a mutation in CYR1 (A5627T corresponding to a K1876M substitution near the end of the catalytic domain in adenylate cyclase which eliminates glucose- and acidification-induced cAMP signalling and delays

References:

(2000) Enzyme Microb Technol 26:706-714

W303

Genotype: MATa/MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} [phi+]

Notes: W303 also contains a bud4 mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In addition, the original W303 strain contains the rad5-535 allele. As S288c, W303 has an allelic variant ofMIP1 which increases petite frequency. The W303 genome was sequenced at the Sanger Institute. References: W303 constructed by Rodney Rothstein (see from RR and Stephan Bartsch).

bud4 info: Voth et al. (2005) Eukaryotic Cell, 4:1018-28.

rad5-535

info: Fan et al. (1996) Genetics 142:749

W303-1A

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