GE-Gel Filtration (Size Exclusion Chromatography)分子筛

GE, AKTA, 蛋白质纯化,分子筛

GE Healthcare

Gel filtration

Principles and Methods

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GE, AKTA, 蛋白质纯化,分子筛

Handbooks

from GE Healthcare

GST Gene Fusion System Handbook 18-1157-58

Affinity Chromatography Principles and Methods 18-1022-29

Antibody Purification Handbook 18-1037-46

Ion Exchange Chromatography and Chromatofocusing Principles and Methods 11-0004-21

Cell Separation Media

Methodology and Applications 18-1115-69

Purifying Challenging Proteins Principles and Methods 28-9095-31

Isolation of mononuclear cells Methodology and Applications 18-1152-69

High-throughput Process Development with PreDictor Plates Principles and Methods 28-9403-58

Gel Filtration

Principles and Methods 18-1022-18

Recombinant Protein Purification Handbook Principles and Methods 18-1142-75

Hydrophobic Interaction and Reversed Phase Chromatography Principles and Methods 11-0012-69

2-D Electrophoresis using immobilized pH gradients Principles and Methods 80-6429-60

Microcarrier Cell Culture Principles and Methods 18-1140-62

Nucleic Acid Sample Preparation for Downstream Analyses Principles and Methods 28-9624-00

Strategies for Protein Purification Handbook 28-9833-31

GE, AKTA, 蛋白质纯化,分子筛

Gel filtration

Principles and Methods

GE, AKTA, 蛋白质纯化,分子筛

Contents

Introduction...............................................................................................................................................7Chapter.1.

Gel.filtration.in.practice..........................................................................................................................9

Introduction .....................................................................................................................................................................9Purification by gel filtration ......................................................................................................................................9

Group separation .............................................................................................................................................10High-resolution fractionation .....................................................................................................................11Rapid purity check and screening ............................................................................................................12Resolution in gel filtration .............................................................................................................................12Sample volume and column dimensions ..............................................................................................12Media selection ...........................................................................................................................................................14Sample and buffer preparation ..........................................................................................................................17

Sample buffer composition .........................................................................................................................17Sample concentration and viscosity .......................................................................................................17Sample volume ..................................................................................................................................................19Buffer composition .........................................................................................................................................19Denaturing (chaotropic) agents and detergents ...............................................................................19Column and media preparation .........................................................................................................................20Sample application ...................................................................................................................................................21Elution and flow rates ..............................................................................................................................................21

Controlling flow .................................................................................................................................................23Method development for high resolution fractionation ..........................................................................23Maintenance of gel filtration columns .............................................................................................................24Equipment selection .................................................................................................................................................24Scaling up ......................................................................................................................................................................24BioProcess Media for large-scale production...............................................................................................25Troubleshooting ..........................................................................................................................................................26Chapter.2.

Superdex:.the.first.choice.for.high.resolution,.short.run.times,.and.high.recovery..................31

Separation options ....................................................................................................................................................33Separation examples ........................................................................................................ …… 此处隐藏：34761字，全部文档内容请下载后查看。喜欢就下载吧 ……