中山大学2004级细胞生物学期末试卷

04级细胞期末考题 填空

真核生物DNA损伤后最重要的反应是—— 细胞程序性死亡可以——、——、——

Golgi体形成的两个模型：膜泡转运模型、潴泡成熟模型

目前没有发现在中间纤维上运动的发动机蛋白，可能是因为：中间纤维无极性 True/False

一个含有核输入信号和核输出信号的蛋白质合成后将会成为穿梭蛋白 (T) 在分裂中……只有星微管是旁观者

Deacetylation of histone tails allows nucleosomes to pack together into tigh ter arrays, which usually reduces gene expression. (T) 没有内膜系统，真核细胞不能生存

myosinII 的唯一作用是肌收缩 （F 还有胞质分裂） 选择

Which of the following proteins would not be found in the smooth endoplasmic reticulum?（ ）

a. Ca2+-pumping enzymes b. cytochrome P450 c. glucose 6-phosphatase d. signal peptidase

If you compared the proteins in a cis Golgi compartment with those in a trans Golgi compartment, you would find:（ ）

a. the proteins in the two compartments are identical.

b. the proteins in the cis compartment are glycosylated and contain modified amino acids, whereas those in the trans compartment are not modified.

c. the proteins in the cis compartment are glycosylated, whereas those in the trans compartment are glycosylated and contain modified amino acids.

d. the proteins of the cis compartment are shorter than those of the trans compartment.

Which type of vesicle of the trans Golgi network would be most likely to carry hormones destined for regulated secretion?（ ） a. lysosomal vesicles b. clathrin-coated vesicles c. non-clathrin-coated vesicles d. all of the above

If you treated cells with a drug that interferes with microtubules, such as colchicine, which of the following would result?（ ） a. Cell shape would be disrupted. b. Mitosis and meiosis would not occur.

c. The intracellular location of organelles would be disrupted. d. All of the above would result.

First you dissolve the membrane from an intact flagellum, using the detergent Triton X-100. Next you soak the axoneme in a solution containing EDTA, which removes the Mg2+. What remains of the axoneme after these treatments?（ ） a.peripheral tubules only

b. peripheral tubules and central tubules, but no side arms or ATPase activity c. peripheral tubules, central tubules, side arms, and ATPase activity

d. peripheral tubules, central tubules, side arms, ATPase activity, and a Membrane 简答：

好像是答糖基化的作用（列举3个）

什么是APC，它在cell cycle的调控中起什么作用 如何理解着丝粒是细胞的保护神

Nocodazole阻止微管的聚合，这样可以得到同步化的细胞。nocodazole处理的细胞停留在cell cycle的哪个地方？ 其作用机制是什么？

How could you explain each of the following experimental observation? Mutations that inactivate the

main protein phosphatase used to catalyze protein dephosphorylations cause a long delay in the recon

struction of the nuclear envelope that normally take place at the end of mitosis. Assume liver and kidney tissues from the same mouse each contain about 10,000 species of cytosolic m

RNA, but only about 25% of these are common to the two tissues. One possible explanation for the dat

a is that differential transcription occurs in liver and kidney nuclei. What is another possible exp lanation?

（They undergo different translation process.)

用微注射注入myosin antibody对后期的染色体分裂有何影响？对胞质分裂有何影响？其作用机制？

A single nucleosome is 11 nm long and contains 146 bp of DNA(0.34nm/bp). What packing

ration (DNA le

ngth to nucleosome length) has been achieved by wrapping DNA a round the histone octamer? Assuming t

hat 3 are an additional 54bp of extended DNA in the linker between nucleosomes, how condensed is \

ads-on-a-string\DNA relative to fully extended DNA?What fraction of the 10,000 fold condensation th

at occurs mitosis does this first level of packing represent? 综合问答：

比较裂殖酵母、芽殖酵母和哺乳动物细胞周期调控的异同 题型变换：

Although the lengths of all phases of the cycle are variable to some extent,by far the greatest vari

ation occurs in the duration of G1. Explain your answer.