Western Blotting Transfer Buffer（ Western Blot 注意！！！）

Western Blotting Transfer Buffer 最近接触Western Blotting实验，在百度上搜了一堆的Western Blot protocol， 结果出问题了，被老板鄙视了一顿。

按照这个所谓的超详细步骤，就像买了哪些脑白金一样，起码在转膜用的transfer buffer 这个关键点上，错的离谱了！！！

不信，自己在Bing.com 上搜 Western Blotting Transfer Buffer！！！

先通用的Western Blotting Transfer Buffer 配方应该是：

Transfer Buffer (for Western blotting) - 1L 25 mM Tris, 192 mM glycine, 20% methanol

1X Transfer Buffer Reagents needed: 10X Transfer Buffer Reagents needed: 14.4 g glycine 3.03 g Tris base 200 ml methanol 0.8 L ddH2O 144.1g glycine 30.2 g Tris base - methanol http://westernblot.org/western-blot-protocol/western-blot-protocol-buffers/ http://www.bio.davidson.edu/Courses/Molbio/Protocols/western.html http://www.cytographica.com/lab/solutions/transfer.htm

http://www.thelabrat.com/protocols/WesternTransferBuffer.shtml

Thermo 官网上公布的转膜buffer 配方

http://www.piercenet.com/method/western-blot-transfer-methods