改性蒙脱石作为抗生素对断奶仔猪肠道菌群的影响

Animal Feed Science and Technology 198(2014)257–262Contents lists available at ScienceDirect

Animal Feed Science and

Technology journal homepage:

3592f506168884868662d60e/locate/anifeedsci Effects of cetylpyridinium-montmorillonite,as alternative to

antibiotic,on the growth performance,intestinal micro?ora

and mucosal architecture of weaned pigs

Y.L.Ke,L.F.Jiao,Z.H.Song,K.Xiao,3592f506168884868662d60ei,J.J.Lu ?,C.H.Hu ??

Animal Science College,Zhejiang University;The Key Laboratory of Molecular Animal Nutrition,Ministry of Education,

Hangzhou 310058,China a r t i c l e i n f o Article history:

Received 7July 2014

Received in revised form 8October 2014

Accepted 9October 2014Keywords:

Antibiotic

Cetylpyridinium-montmorillonite

Intestinal micro?ora

Mucosal architecture

Weaned pigs a b s t r a c t Cetylpyridinium-montmorillonite (CP-Mt)was used as an alternative to chlortetracycline on growth performance,intestinal micro?ora and morphology in weaned pigs.A total of 150piglets (Duroc ×Landrace ×Yorkshire,weaned at 21±1d age)were allotted to ?ve groups:(1)control;(2)control +0.5g/kg CP-Mt;(3)control +1.0g/kg CP-Mt;(4)control +1.5g/kg CP-Mt;(5)control +75mg/kg chlortetracycline.Each treatment has six pens with ?ve pigs per pen.The feeding trial lasted three weeks.The results showed that incremental CP-Mt inclusion in the diet increased average daily gain (linear P=0.013;quadratic P=0.034),decreased Escherichia coli and Streptococcus suis in jejunal contents linearly (P=0.004and P=0.006)and quadratically (P=0.014and P=0.009),decreased E.coli and Streptococcus suis in colon linearly (P=0.005and P=0.001)and quadratically (P=0.007and P=0.004),increased

villus height (linear P<0.001;quadratic P=0.001)and the ratio of villus height and crypt

depth (linear P<0.001;quadratic P=0.001),reduced plasma diamine oxidase (DAO)(linear

P=0.000;quadratic P=0.001),and increased jejunal mucosa DAO (linear P=0.006;quadratic

P=0.002).The piglets fed 1.0g/kg and 1.5g/kg CP-Mt did not differ from those fed chlorte-

tracycline (P>0.05)in growth performance,intestinal micro?ora and mucosal architecture.

The results indicated that CP-Mt could be a good candidate as an antibiotic alternative in

weaned pigs.

?2014Elsevier B.V.All rights reserved.1.Introduction

Early-weaned piglets commonly encounter low feed intake,body weight loss and post-weaning diarrhea (Hu et al.,2013b;McLamb et al.,2013;Smith et al.,2010).There is a large number of evidence showing that weaning disturbs intestinal microbiota and impairs intestinal morphology (Hu et al.,2012b,2013c;Jiao et al.,2014).Antibiotic is widely used to reduce enteric infections and the occurrence of pathogens able to adhere to intestinal mucosa (Kil and Stein,2010).The increased use of antibiotics has given rise to a concern of the development of resistant pathogenic bacterial strains and residual Abbreviations:ADFI,average daily feed intake;ADG,average daily gain;SEM,standard error of the mean;CP-Mt,cetylpyridinium-montmorillonite;DAO,diamine oxidase.

?Corresponding author.??Corresponding author.Tel.:+8657186961553;fax:+8657186961553.

E-mail addresses:jjlu@3592f506168884868662d60e (J.J.Lu),chhu@3592f506168884868662d60e (C.H.Hu).

3592f506168884868662d60e/10.1016/j.anifeedsci.2014.10.010

0377-8401/?2014Elsevier B.V.All rights reserved.

258Y.L.Ke et al./Animal Feed Science and Technology198(2014)257–262

contamination of the food chain with antibiotic(Vondruskova et al.,2010).In2006,the use of antibiotics as growth promoters was forbidden in the EU.

Special attention has been paid to natural clay minerals,which were not only used as antibiotic alternatives(Philip,2013; Song et al.,2012),but also used as carriers of antibacterial agent(Hu and Xia,2006;?zdemir et al.,2010).Montmorillonite (MMT),an aluminosilicate clay,has been generally used as an antimicrobial carrier due to its large speci?c surface area and high cation exchange capacity.The recent studies are mainly focused on immobilization of inorganic antimicrobial cations such as Cu2+and ZnO onto montmorillonite(Hu et al.,2012a,2013a;Song et al.,2013b).

Immobilization of organic cations with antibacterial activity has some advantages compared to that of inorganic cations (Herrera et al.,2000).Cetylpyridinium chloride as a quaternary ammonium salt is such an agent used in antiseptic solutions, and?nds a wide range of antibacterial applications in oral rinses and throat lozenges,in the meat industry for the decon-tamination of carcasses,and in the further processing of poultry,pork,and beef for the control of microbial growth(?zdemir et al.,2013).Previous investigations demonstrated that cetylpyridinium-montmorillonite(CP-Mt)exhibited strong antibac-terial activity for pathogenic bacteria,such as Salmonella enteritidis and Staphylococcus aureus(Herrera et al.,2000;?zdemir et al.,2013).

However,there are no data on the effects of CP-Mt in vivo.In this experiment,we hypothesized that CP-Mt could be used as alternative to chlortetracycline on growth performance,intestinal micro?ora and mucosal architecture in weaned pigs.

2.Materials and methods

2.1.Materials

The montmorillonite(Chifeng WHTB Mining Co.,LTD,Chifeng,China)content was99.0%.The cation exchange capacity (CEC)was1.30moL(+)/kg montmorillonite.The cetylpyridinium-montmorillonite(CP-Mt)was synthesized according to the method of Herrera et al.(2000)and?zdemir et al.(2013).Montmorillonite was hydrated with water to which cetylpyridinium chloride was added at an amount of0.5times the CEC of the montmorillonite.The resulting mixture was then agitated and allowed to exchange for24h.The CP-Mt was then separated by centrifugation and washed under agitation for24h with 100mL deionized water.The washed material was …… 此处隐藏：22909字，全部文档内容请下载后查看。喜欢就下载吧 ……