Ⅱ型糖尿病大鼠血糖波动模型的初步建立及其特点

Ⅱ型糖尿病大鼠血糖波动模型的初步建立及其特点

朱延涛;金蓉家;钟杰敏;李守业;徐志伟;寿旗扬;蔡月琴;王辉

【期刊名称】《实验动物与比较医学》

【年(卷),期】2012(032)006

【摘要】目的建立Ⅱ型糖尿病大鼠血糖波动模型,同时观察血糖波动对糖尿病大鼠胰腺损伤情况.方法用30 mg/kg链脲佐菌素诱导SD大鼠糖尿病模型,错时腹腔注射0.375 g/kg葡萄糖和皮下注射超短效胰岛素制备糖尿病血糖波动模型,观察各组大鼠糖化血红蛋白(HbAlc)、血清游离脂肪酸(FFA)、胰岛素(INS)、胰腺中Bax和Bcl-2含量.结果模型波动组和模型组的血糖显著高于正常组(P＜0.01),模型波动组和模型组中HbAlc、FFA、INS含量显著高于正常组(P＜0.01),与模型组相比,模型波动组中HbAlc、FFA、INS含量显著高于模型组合量(P＜0.01)；模型波动组胰腺中Bax含量显著升高(P＜0.01)、Bcl-2含量显著下降(P＜0.01).结论每日两次错时注射胰岛素和葡萄糖可以成功建立大鼠糖尿病血糖波动模型,且血糖波动能加重胰腺损伤程度.%Objective To establish the animal model of diabetic rats with fluctuant high blood glucose and observe the effects of fluctuant high blood glucose on pancreas in diabetic rats. Methods The diabetic rats model was developed by injection with streptozotocin (STZ) 30 mg/kg, regularly staggered insulin 1U and glucose 0.375 mg/kg administration were used to set up blood glucose fluctuation model. General symptoms were observed and the data of FFA、INS、HbAlc in blood as well as the Bax and Bcl-2 in pancreas were measured at the thirteen week. Results (1) The typical